Site-directed mutagenesis of large plasmids.
نویسندگان
چکیده
A protocol combining recombination PCR and long-distance PCR is demonstrated to be highly accurate and rapid for site-directed mutagenesis of large (> 10 kb) plasmids. Application of this protocol to the generation of mutant rabies virus glycoproteins expressed by the baculovirus/insect cell system illustrates the usefulness of this approach in facilitating structure-function relationships in this important eukaryotic expression system.
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ورودعنوان ژورنال:
- BioTechniques
دوره 25 6 شماره
صفحات -
تاریخ انتشار 1998